Journal: International Journal of Molecular Sciences

Article Title: Semi-Biosynthetic Production of Surface-Binding Adhesive Antimicrobial Peptides Using Intein-Mediated Protein Ligation

doi: 10.3390/ijms232315202

Figure Lengend Snippet: Fed-batch fermentation of E. coli BL21 (DE3)-pET21b-HNG. ( a ) E. coli fed-batch fermentation kinetics for the production of HNG. Cells were cultivated in batch mode until the initially present glucose was depleted. After 5 h, glucose feeding was initiated at a predefined rate. At an OD 600 of 62, protein expression was induced with 0.1 mM IPTG. Samples were collected to monitor cell OD 600 (black) and medium glucose content (red). ( b ) Culture samples collected at induction times of 9, 12, 15, 18, 21, and 24 h were analyzed using SDS-PAGE and Western blotting with an anti-His antibody. Lane M: protein molecular weight marker; Lane W: whole cell lysate; Lane S: soluble fraction; Lane I: insoluble fraction. The target protein is indicated by arrows.

Article Snippet: E. coli strains DH5α and BL21 (DE3) (RBC Bioscience, Taipei, Taiwan) were used as hosts for cloning and HNG fusion protein expression, respectively. pET21b (Novagen, Madison, WI, USA) was used to construct expression plasmids.

Techniques: Expressing, SDS Page, Western Blot, Molecular Weight, Marker

Journal: AMB Express

Article Title: Genomic and molecular characterization of a novel quorum sensing molecule in Bacillus licheniformis

doi: 10.1186/s13568-017-0381-6

Figure Lengend Snippet: ComX bioactivity in bacterial culture supernatants from late exponential phase B. licheniformis NCIMB 8874 and E. coli BL21 ectopically expressing a srfA - lacZ fusion, as determined through a β-galactosidase reporter ( B. subtilis JRL293). Data were collected from three independent experiments which were performed in triplicate. The constant blank (water) has been subtracted from all calculated values. The details of the tested culture supernatants are presented in the table

Article Snippet: Fig. 6 ComX bioactivity in bacterial culture supernatants from late exponential phase B. licheniformis NCIMB 8874 and E. coli BL21 ectopically expressing a srfA - lacZ fusion, as determined through a β-galactosidase reporter ( B. subtilis JRL293).

Techniques: Expressing

Journal: bioRxiv

Article Title: Expression of Pokeweed Antiviral Protein Isoform S1 (PAP-S1) and of Ricin-A-Chain/PAP-S1 novel fusion protein (RTA/PAP-S1) in Escherichia coli and their comparative inhibition of protein synthesis in vitro

doi: 10.1101/137919

Figure Lengend Snippet: Protein Purification. a) Stained gel of RTA/PAP-S1R68G from third wash (sample W) and from pooled eluted fraction (sample E) at 60kDa (red arrow) and of PAP-S1R68G at 30kDa (green arrow), all from native environment, after buffer exchange. The purity of sample W was 65%, of sample E 55%, and of PAP-S1R68G 60%. b) Close up and Western Blot of sample W and E. A double band is present and may be due to degradation of the protein or as a result of an unwanted recombination by E. coli cells.

Article Snippet: The E. coli pT7 expression vector and E. coli strains BL21(DE3) were purchased from Proteogenix.

Techniques: Protein Purification, Staining, Buffer Exchange, Western Blot